产品货号：
Z22023
中文名称：
Nephrin/NPHS1抗体
英文名称：
Anti-Nephrin/NPHS1 Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-Nephrin/NPHS1 Antibody |
| 指标别名 | NPHS1adhesion molecule;nephrin;CNF;NPHN;nephrin |
| 克隆性 | Polyclonal |
| 检验物种 | human,mouse,rat |
| 应用范围 | WB,IHC |
| 基因名称 | NPHS1 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | E.coli-derived human Nephrin recombinant protein (Position:V1084-V1241).Human Nephrin shares 79.9% and 75.3% amino acid (aa) sequence identity with mouse and rat Nephrin,respectively. |
| 实际分子量 | 135KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | NPHS1,also called Nephrin,is mapped to 19q13.12.This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney.It gene is primarily expressed in renal tissues,and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes.The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine.A defect in the gene for nephrin,NPHS1,is associated with congenital nephrotic syndrome of the Finnish type and causes massive amounts of protein to be leaked into the urine,orproteinuria.NPHS1 is also required for cardiovascular development. |
| Uniprot ID | O60500 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P).*Blocking peptide可以联系我们购买。 |
| 推荐稀释比 | Western blot (WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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